ppe anti il 7rα  (Bio X Cell)

Journal: Nature Communications

Article Title: Maternal asthma imprints fetal lung ILC2s via glucocorticoid signaling leading to worsened allergic airway inflammation in murine adult offspring

doi: 10.1038/s41467-025-55941-8

Figure Lengend Snippet: a Flow cytometric analysis of fetal lungs from asthmatic or control mothers (day 18 of fetal life); ILC2s were stimulated with PMA/ionomycin for 4 h, and cytokine production was assessed by intracellular staining. b Model of maternal anti-IL-7Rα treatment in late pregnancy. Female mice developed asthma during pregnancy (Fig. ) and were treated intravenously with isotype or anti-IL-7Rα antibodies on days 14, 16, and 18 of gestation. c Adult offspring of antibody-treated asthmatic mothers received PBS or HDM intranasally (Fig. ). Changes in eosinophil and ILC2 cell numbers in the lungs of adult offspring; ILC2s were stimulated with PMA/ionomycin for 4 h, and cytokine production was assessed by intracellular staining. In ( a , c ), data are representative of two independent experiments [( a ), intracellular staining was evaluated once], each experiment with one pregnant dam per group, or pooled from two independent experiments, each experiment with one or two pregnant dams. Sample sizes were as follows: a PBS: n = 7, OVA: n = 6; c OVA(Iso)-PBS: n = 9, OVA(anti-IL-7Rα)-PBS: n = 6, OVA(Iso)-HDM: n = 14, OVA(anti-IL-7Rα)-HDM: n = 8. In ( a , c ), each dot represents an individual mouse. In ( a , c ), data are presented as the mean ± SEM. * p < 0.05; **** p < 0.0001; ns, not significant [unpaired two-tailed Student’s t -test].

Article Snippet: For depletion of fetal ILC2, IL-7Ra blockade was performed by intravenous injection of 200 μg isotype control (Bio X Cell, BE0089) or anti-IL-7Rα antibody (Bio X Cell, BE0065) into pregnant female mice on days 14, 16, and 18 of gestation.

Techniques: Control, Staining, Two Tailed Test

Journal: Nature communications

Article Title: Maternal asthma imprints fetal lung ILC2s via glucocorticoid signaling leading to worsened allergic airway inflammation in murine adult offspring.

doi: 10.1038/s41467-025-55941-8

Figure Lengend Snippet: Fig. 3 | Pulmonary immune changes in embryonic life affect adult allergic responses. a Flow cytometric analysis of fetal lungs from asthmatic or control mothers (day 18 of fetal life); ILC2s were stimulated with PMA/ionomycin for 4 h, and cytokine production was assessed by intracellular staining. b Model of maternal anti-IL-7Rα treatment in late pregnancy. Female mice developed asthma during pregnancy (Fig. 1a) and were treated intravenously with isotype or anti-IL- 7Rα antibodies on days 14, 16, and 18 of gestation. c Adult offspring of antibody- treated asthmatic mothers received PBS or HDM intranasally (Fig. 1a). Changes in eosinophil and ILC2 cell numbers in the lungs of adult offspring; ILC2s were

Article Snippet: For depletion of fetal ILC2, IL-7Ra blockade was performed by intravenous injection of 200 μg isotype control (BioXCell, BE0089) or antiIL-7Rα antibody (Bio X Cell, BE0065) into pregnant female mice on days 14, 16, and 18 of gestation.

Techniques: Control, Staining